Parameters Targets File: Non Targets File: Primers File: mergedPrimersN3.ml=20.Ml=25.txt Primers Output File: Min Primer Length: 20 Max Primer Length: 25 Min Product Length: 75 Max Product Length: 200 Forward Primer: NONE Reverse Primer: NONE Range of positions for forward primers: 0 - 100000 Range of positions for reverse primers: 0 - 100000 Targets Mask: 11 Non Targets Mask: NONE Degeneracy Mask: 1 Number of Source Sequences: 1 Min Coverage Targets: 100.00 Max Coverage Non Targets: 0.00 Max Self Score: 800.00 Max End Score: 300.00 Min GC Content: 25.00 Max GC Content: 75.00 GC Clamp: 0 Max Poly-X: 5 Primer Concentration: 0.000000800 Template Concentration: 0.000000000 Salt Concentration: 0.05000 Salt Correction Method: 1 Targets Min Melting Temp : 40.00 Targets Max Melting Temp: 100.00 Non Targets Max Melting Temp: 40.00 Non Targets Delta Temp: 0.00 Max Pair Temperature Difference: 40.00 Primers Label: N3 Dependent stats Sequence for ranking: NONE Targets: 0 Non Targets: 0 Load time: 0 Running process for forward primers Build tables time: 1 Forward_N3_S1_3 TGTGCCCCTTCCGAGGATTCTTCCC 312 42.25 59.01 66.03 23.64 100.00% 0.00% Forward_N3_S1_4 GTGCCCCTTCCGAGGATTCTTCCCT 314 40.74 60.18 67.37 27.81 100.00% 0.00% Forward_N3_S1_10 CTTCCGAGGATTCTTCCCTTTTCAC 326 41.07 53.70 60.65 27.25 100.00% 0.00% Forward_N3_S1_11 TTCCGAGGATTCTTCCCTTTTCACA 327 41.48 54.50 59.96 27.25 100.00% 0.00% Forward_N3_S1_12 TCCGAGGATTCTTCCCTTTTCACAA 328 41.49 54.64 59.93 27.25 100.00% 0.00% Forward_N3_S1_14 TTCTTCCCTTTTCACAAGGACAAT 329 42.64 55.81 57.13 37.83 100.00% 0.00% Forward_N3_S1_15 TTCTTCCCTTTTCACAAGGACAATG 330 45.11 56.97 58.17 36.85 100.00% 0.00% Forward_N3_S1_20 TGCTTGGTCGAGTAGCAGTTGCTTT 571 46.23 61.34 64.08 35.97 100.00% 0.00% Forward_N3_S1_21 GCTTGGTCGAGTAGCAGTTGCTTTG 573 47.49 61.54 64.11 38.98 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% Forward_N3_S1_43 ATAGTTACTTTCTGTGGGTTAGACA 1368 42.33 50.49 56.25 27.23 100.00% 0.00% Forward_N3_S1_44 TAGTTACTTTCTGTGGGTTAGACAA 1369 42.20 50.46 56.42 22.66 100.00% 0.00% Forward_N3_S1_45 AGTTACTTTCTGTGGGTTAGACAAT 1370 44.01 52.12 57.72 24.68 100.00% 0.00% Forward_N3_S1_46 GTTACTTTCTGTGGGTTAGACAATG 1371 44.52 52.19 57.46 30.65 100.00% 0.00% Forward_N3_S1_47 TTACTTTCTGTGGGTTAGACAATGA 1379 42.50 50.37 56.15 26.59 100.00% 0.00% Forward_N3_S1_48 TACTTTCTGTGGGTTAGACAATGAA 1369 41.31 50.44 56.51 28.13 100.00% 0.00% Forward_N3_S1_50 TTTCTGTGGGTTAGACAATGAACCT 1376 41.91 53.29 58.74 23.37 100.00% 0.00% Forward_N3_S1_51 TTCTGTGGGTTAGACAATGAACCTG 1377 45.33 54.38 59.65 26.10 100.00% 0.00% Forward_N3_S1_52 TCTGTGGGTTAGACAATGAACCTGG 1378 48.11 56.29 61.39 25.57 100.00% 0.00% Forward_N3_S1_53 CTGTGGGTTAGACAATGAACCTGGA 1379 49.51 57.52 62.52 27.17 100.00% 0.00% Forward_N3_S1_54 TGTGGGTTAGACAATGAACCTGGAT 1380 46.90 55.19 60.38 26.35 100.00% 0.00% Forward_N3_S1_55 TGGGTTAGACAATGAACCTGGATCG 1382 47.42 55.83 61.07 22.43 100.00% 0.00% Forward_N3_S1_56 GGGTTAGACAATGAACCTGGATCGG 1391 49.98 57.89 62.34 25.81 100.00% 0.00% Forward_N3_S1_57 GGTTAGACAATGAACCTGGATCGGG 1391 53.24 59.45 62.21 27.92 100.00% 0.00% Forward_N3_S1_58 GTTAGACAATGAACCTGGATCGGGA 1396 43.35 57.83 60.72 27.60 100.00% 0.00% Forward_N3_S1_61 AGACAATGAACCTGGATCGGGAAAT 1388 53.33 61.16 61.94 35.32 100.00% 0.00% Forward_N3_S1_62 ACAATGAACCTGGATCGGGAAATTG 1390 52.51 60.51 61.25 36.28 100.00% 0.00% Forward_N3_S1_63 CAATGAACCTGGATCGGGAAATTGG 1391 53.17 60.73 61.42 34.10 100.00% 0.00% Forward_N3_S1_64 AATGAACCTGGATCGGGAAATTGGC 1392 54.89 62.32 63.01 36.39 100.00% 0.00% Forward_N3_S1_65 ATCGGGAAATTGGCCGGATG 1398 47.77 58.87 61.65 25.37 100.00% 0.00% Forward_N3_S1_66 CTGGATCGGGAAATTGGCCGGATGG 1399 58.04 65.24 66.92 34.64 100.00% 0.00% Forward_N3_S1_67 TGGATCGGGAAATTGGCCGGATGGT 1400 57.28 64.79 66.54 37.85 100.00% 0.00% Forward_N3_S1_68 GGATCGGGAAATTGGCCGGATGGTT 1389 44.00 64.49 66.87 33.48 100.00% 0.00% Forward_N3_S1_69 ATCGGGAAATTGGCCGGATGGTTCC 1393 42.91 60.83 66.92 33.29 100.00% 0.00% Forward_N3_S1_76 CATTGGGTTTATGCCCAAGTAA 1433 52.48 55.56 56.66 25.26 100.00% 0.00% Forward_N3_S1_77 ACATTGGGTTTATGCCCAAGTAACA 1422 48.61 56.60 59.69 26.95 100.00% 0.00% Forward_N3_S1_78 CATTGGGTTTATGCCCAAGTAACAG 1424 51.13 56.90 59.60 27.78 100.00% 0.00% Generate primers time: 0 Running process for reverse primers Build tables time: 0 Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% Reverse_N3_S1_9 CCAATGTTGGAACCATCCGGCCAAT 1387 42.02 58.62 65.29 36.48 100.00% 0.00% Reverse_N3_S1_12 AACCATCCGGCCAATTTCCCGATCC 1377 51.45 64.12 66.92 30.08 100.00% 0.00% Reverse_N3_S1_13 ACCATCCGGCCAATTTCCCGATCCA 1376 54.19 64.05 66.69 29.19 100.00% 0.00% Reverse_N3_S1_14 CCATCCGGCCAATTTCCCGATCCAG 1375 53.99 63.51 66.07 30.78 100.00% 0.00% Reverse_N3_S1_15 CATCCGGCCAATTTCCCGATCCAGG 1374 54.91 64.39 66.92 28.08 100.00% 0.00% Reverse_N3_S1_16 ATCCGGCCAATTTCCCGATCCAGGT 1366 52.50 65.20 67.58 26.64 100.00% 0.00% Reverse_N3_S1_17 TCCGGCCAATTTCCCGATCCAGGTT 1368 51.71 64.27 67.28 27.90 100.00% 0.00% Reverse_N3_S1_18 CGGCCAATTTCCCGATCCAGGTTCA 1370 54.26 64.51 66.05 24.54 100.00% 0.00% Reverse_N3_S1_19 GGCCAATTTCCCGATCCAGGTTCAT 1369 56.75 63.54 64.98 23.53 100.00% 0.00% Reverse_N3_S1_20 GCCAATTTCCCGATCCAGGTTCATT 1368 54.71 62.35 63.00 24.41 100.00% 0.00% Reverse_N3_S1_21 CCAATTTCCCGATCCAGGTTCATTG 1367 53.66 61.28 61.94 23.64 100.00% 0.00% Reverse_N3_S1_22 CAATTTCCCGATCCAGGTTCATTGT 1366 52.73 60.50 61.16 25.01 100.00% 0.00% Reverse_N3_S1_23 AATTTCCCGATCCAGGTTCATTGTC 1365 52.54 60.40 60.99 22.87 100.00% 0.00% Reverse_N3_S1_24 ATTTCCCGATCCAGGTTCATTGTCT 1364 53.13 60.89 61.56 23.10 100.00% 0.00% Reverse_N3_S1_25 TTTCCCGATCCAGGTTCATTGTCTA 1363 50.09 58.34 59.09 18.77 100.00% 0.00% Reverse_N3_S1_26 TTCCCGATCCAGGTTCATTGTCTAA 1353 40.69 54.37 60.55 22.59 100.00% 0.00% Reverse_N3_S1_27 TCCCGATCCAGGTTCATTGTCTAAC 1357 48.64 54.90 61.11 19.15 100.00% 0.00% Reverse_N3_S1_28 CCCGATCCAGGTTCATTGTCTAACC 1359 49.62 56.91 63.18 22.36 100.00% 0.00% Reverse_N3_S1_29 CCGATCCAGGTTCATTGTCTAACCC 1359 49.99 55.94 62.14 21.86 100.00% 0.00% Reverse_N3_S1_30 CGATCCAGGTTCATTGTCTAACCCA 1358 46.98 53.14 59.62 26.79 100.00% 0.00% Reverse_N3_S1_31 GATCCAGGTTCATTGTCTAACCCAC 1357 46.93 53.17 59.71 28.55 100.00% 0.00% Reverse_N3_S1_32 ATCCAGGTTCATTGTCTAACCCACA 1358 47.67 54.33 61.19 22.52 100.00% 0.00% Reverse_N3_S1_33 TCCAGGTTCATTGTCTAACCCACAG 1355 47.90 54.05 60.48 26.68 100.00% 0.00% Reverse_N3_S1_34 CCAGGTTCATTGTCTAACCCACAGA 1354 49.24 55.51 61.98 21.28 100.00% 0.00% Reverse_N3_S1_35 CAGGTTCATTGTCTAACCCACAGAA 1353 45.02 52.69 59.42 23.24 100.00% 0.00% Reverse_N3_S1_36 AGGTTCATTGTCTAACCCACAGAAA 1353 43.43 51.84 58.64 19.30 100.00% 0.00% Reverse_N3_S1_37 GGTTCATTGTCTAACCCACAGAAAG 1355 43.01 53.22 59.72 27.64 100.00% 0.00% Reverse_N3_S1_38 GTTCATTGTCTAACCCACAGAAAGT 1350 40.53 51.58 58.29 24.52 100.00% 0.00% Reverse_N3_S1_39 TTCATTGTCTAACCCACAGAAAGTA 1345 40.23 48.68 56.15 21.99 100.00% 0.00% Reverse_N3_S1_40 TCATTGTCTAACCCACAGAAAGTAA 1348 40.92 49.40 57.18 27.30 100.00% 0.00% Reverse_N3_S1_41 CATTGTCTAACCCACAGAAAGTAAC 1347 42.46 51.53 58.41 20.68 100.00% 0.00% Reverse_N3_S1_42 TTGTCTAACCCACAGAAAGTAACTA 1345 42.08 49.33 56.60 23.12 100.00% 0.00% Reverse_N3_S1_46 AACTATACTATTGCTTGTCCAAGAG 1325 45.20 54.45 56.27 35.49 100.00% 0.00% Reverse_N3_S1_47 CTATACTATTGCTTGTCCAAGAGAC 1323 45.34 54.29 56.18 37.72 100.00% 0.00% Reverse_N3_S1_48 ATACTATTGCTTGTCCAAGAGACAT 1315 46.12 55.35 57.81 32.99 100.00% 0.00% Reverse_N3_S1_49 TACTATTGCTTGTCCAAGAGACATC 1317 43.65 55.88 58.19 27.03 100.00% 0.00% Reverse_N3_S1_50 ACTATTGCTTGTCCAAGAGACATCA 1316 45.55 57.30 59.59 27.69 100.00% 0.00% Reverse_N3_S1_51 CTATTGCTTGTCCAAGAGACATCAT 1321 44.06 56.02 58.56 27.23 100.00% 0.00% Reverse_N3_S1_52 TATTGCTTGTCCAAGAGACATCATC 1317 43.94 56.33 58.79 28.85 100.00% 0.00% Reverse_N3_S1_53 ATTGCTTGTCCAAGAGACATCATCA 1316 46.74 57.86 60.10 26.92 100.00% 0.00% Reverse_N3_S1_54 TGCTTGTCCAAGAGACATCATCATT 1312 46.30 57.80 59.93 31.94 100.00% 0.00% Reverse_N3_S1_55 GGGCTGGAAACAGCCCTTGGTT 1254 40.34 51.53 65.71 32.69 100.00% 0.00% Reverse_N3_S1_56 GGGCTGGAAACAGCCCTTGGTTT 1253 40.51 52.06 65.60 36.12 100.00% 0.00% Reverse_N3_S1_57 GGGCTGGAAACAGCCCTTGGTTTT 1254 40.51 52.36 65.51 35.39 100.00% 0.00% Reverse_N3_S1_58 GGGCTGGAAACAGCCCTTGGTTTTG 1256 40.26 53.61 65.89 38.94 100.00% 0.00% Reverse_N3_S1_63 CAAAGCAACTGCTACTCGACCAAGC 549 43.58 60.18 64.72 33.32 100.00% 0.00% Reverse_N3_S1_69 CCTTGTGCGAGAGCGAAGGACCAAC 403 40.47 60.76 67.75 28.47 100.00% 0.00% Reverse_N3_S1_71 TGCATTGTCCTTGTGAAAAGGGAAG 308 46.38 59.57 61.11 34.63 100.00% 0.00% Reverse_N3_S1_72 GCATTGTCCTTGTGAAAAGGGAAGA 310 48.19 59.88 61.36 31.78 100.00% 0.00% Reverse_N3_S1_73 CATTGTCCTTGTGAAAAGGGAAGAA 306 46.17 57.44 58.96 34.05 100.00% 0.00% Reverse_N3_S1_75 CTTGTGAAAAGGGAAGAATCCTCGG 294 41.67 53.50 60.98 26.57 100.00% 0.00% Reverse_N3_S1_78 AAAAGGGAAGAATCCTCGGAAGGGG 294 40.95 57.79 64.13 28.36 100.00% 0.00% Reverse_N3_S1_79 AAGGGAAGAATCCTCGGAAGGGGCA 291 40.56 58.78 64.69 34.35 100.00% 0.00% Reverse_N3_S1_81 AGAATCCTCGGAAGGGGCACAGCGG 293 43.82 63.06 69.91 31.23 100.00% 0.00% Generate primers time: 0 Forward_N3_S1_3 TGTGCCCCTTCCGAGGATTCTTCCC 312 42.25 59.01 66.03 23.64 100.00% 0.00% - Reverse_N3_S1_69 CCTTGTGCGAGAGCGAAGGACCAAC 403 40.47 60.76 67.75 28.47 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_9 CCAATGTTGGAACCATCCGGCCAAT 1387 42.02 58.62 65.29 36.48 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_12 AACCATCCGGCCAATTTCCCGATCC 1377 51.45 64.12 66.92 30.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_29 ATGATGTCTCTTGGACAAGCAATAG 1347 48.80 57.08 59.30 33.62 100.00% 0.00% - Reverse_N3_S1_14 CCATCCGGCCAATTTCCCGATCCAG 1375 53.99 63.51 66.07 30.78 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_9 CCAATGTTGGAACCATCCGGCCAAT 1387 42.02 58.62 65.29 36.48 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_12 AACCATCCGGCCAATTTCCCGATCC 1377 51.45 64.12 66.92 30.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_13 ACCATCCGGCCAATTTCCCGATCCA 1376 54.19 64.05 66.69 29.19 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_14 CCATCCGGCCAATTTCCCGATCCAG 1375 53.99 63.51 66.07 30.78 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_30 TGATGTCTCTTGGACAAGCAATAGT 1348 48.00 55.90 58.05 36.37 100.00% 0.00% - Reverse_N3_S1_15 CATCCGGCCAATTTCCCGATCCAGG 1374 54.91 64.39 66.92 28.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_9 CCAATGTTGGAACCATCCGGCCAAT 1387 42.02 58.62 65.29 36.48 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_12 AACCATCCGGCCAATTTCCCGATCC 1377 51.45 64.12 66.92 30.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_13 ACCATCCGGCCAATTTCCCGATCCA 1376 54.19 64.05 66.69 29.19 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_14 CCATCCGGCCAATTTCCCGATCCAG 1375 53.99 63.51 66.07 30.78 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_31 GATGTCTCTTGGACAAGCAATAGTA 1341 47.98 56.35 58.71 36.68 100.00% 0.00% - Reverse_N3_S1_15 CATCCGGCCAATTTCCCGATCCAGG 1374 54.91 64.39 66.92 28.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_9 CCAATGTTGGAACCATCCGGCCAAT 1387 42.02 58.62 65.29 36.48 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_12 AACCATCCGGCCAATTTCCCGATCC 1377 51.45 64.12 66.92 30.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_14 CCATCCGGCCAATTTCCCGATCCAG 1375 53.99 63.51 66.07 30.78 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_32 TCTTGGACAAGCAATAGTATAGTTA 1346 45.23 51.74 53.90 38.28 100.00% 0.00% - Reverse_N3_S1_15 CATCCGGCCAATTTCCCGATCCAGG 1374 54.91 64.39 66.92 28.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_9 CCAATGTTGGAACCATCCGGCCAAT 1387 42.02 58.62 65.29 36.48 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_33 CTTGGACAAGCAATAGTATAGTTAC 1351 48.37 54.04 55.95 38.51 100.00% 0.00% - Reverse_N3_S1_12 AACCATCCGGCCAATTTCCCGATCC 1377 51.45 64.12 66.92 30.08 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_36 AGCAATAGTATAGTTACTTTCTGTG 1359 46.32 52.82 54.22 35.12 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_37 AATAGTATAGTTACTTTCTGTGG 1360 42.57 50.91 51.44 35.16 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_38 CAATAGTATAGTTACTTTCTGTGGG 1360 42.09 50.44 54.76 35.26 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% - Reverse_N3_S1_6 AAACCCAATGTTGGAACCATCCGGC 1391 42.31 59.07 65.37 27.35 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% - Reverse_N3_S1_7 ACCCAATGTTGGAACCATCCGGCCA 1389 44.26 61.31 67.67 33.67 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_39 AATAGTATAGTTACTTTCTGTGGGT 1358 42.17 50.99 54.95 33.15 100.00% 0.00% - Reverse_N3_S1_8 CCCAATGTTGGAACCATCCGGCCAA 1388 43.89 60.07 66.25 34.79 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_43 ATAGTTACTTTCTGTGGGTTAGACA 1368 42.33 50.49 56.25 27.23 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_43 ATAGTTACTTTCTGTGGGTTAGACA 1368 42.33 50.49 56.25 27.23 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_43 ATAGTTACTTTCTGTGGGTTAGACA 1368 42.33 50.49 56.25 27.23 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_43 ATAGTTACTTTCTGTGGGTTAGACA 1368 42.33 50.49 56.25 27.23 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_44 TAGTTACTTTCTGTGGGTTAGACAA 1369 42.20 50.46 56.42 22.66 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_44 TAGTTACTTTCTGTGGGTTAGACAA 1369 42.20 50.46 56.42 22.66 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_44 TAGTTACTTTCTGTGGGTTAGACAA 1369 42.20 50.46 56.42 22.66 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_44 TAGTTACTTTCTGTGGGTTAGACAA 1369 42.20 50.46 56.42 22.66 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_45 AGTTACTTTCTGTGGGTTAGACAAT 1370 44.01 52.12 57.72 24.68 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_45 AGTTACTTTCTGTGGGTTAGACAAT 1370 44.01 52.12 57.72 24.68 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_45 AGTTACTTTCTGTGGGTTAGACAAT 1370 44.01 52.12 57.72 24.68 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_45 AGTTACTTTCTGTGGGTTAGACAAT 1370 44.01 52.12 57.72 24.68 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_46 GTTACTTTCTGTGGGTTAGACAATG 1371 44.52 52.19 57.46 30.65 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_46 GTTACTTTCTGTGGGTTAGACAATG 1371 44.52 52.19 57.46 30.65 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_46 GTTACTTTCTGTGGGTTAGACAATG 1371 44.52 52.19 57.46 30.65 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_47 TTACTTTCTGTGGGTTAGACAATGA 1379 42.50 50.37 56.15 26.59 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_47 TTACTTTCTGTGGGTTAGACAATGA 1379 42.50 50.37 56.15 26.59 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_47 TTACTTTCTGTGGGTTAGACAATGA 1379 42.50 50.37 56.15 26.59 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_48 TACTTTCTGTGGGTTAGACAATGAA 1369 41.31 50.44 56.51 28.13 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_48 TACTTTCTGTGGGTTAGACAATGAA 1369 41.31 50.44 56.51 28.13 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_48 TACTTTCTGTGGGTTAGACAATGAA 1369 41.31 50.44 56.51 28.13 100.00% 0.00% - Reverse_N3_S1_3 CTTGGGCATAAACCCAATGTTGGAA 1408 50.67 55.26 59.28 20.49 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_48 TACTTTCTGTGGGTTAGACAATGAA 1369 41.31 50.44 56.51 28.13 100.00% 0.00% - Reverse_N3_S1_4 TGGGCATAAACCCAATGTTGGAACC 1398 49.03 58.52 62.74 24.31 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_50 TTTCTGTGGGTTAGACAATGAACCT 1376 41.91 53.29 58.74 23.37 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_50 TTTCTGTGGGTTAGACAATGAACCT 1376 41.91 53.29 58.74 23.37 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_51 TTCTGTGGGTTAGACAATGAACCTG 1377 45.33 54.38 59.65 26.10 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_51 TTCTGTGGGTTAGACAATGAACCTG 1377 45.33 54.38 59.65 26.10 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_52 TCTGTGGGTTAGACAATGAACCTGG 1378 48.11 56.29 61.39 25.57 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_52 TCTGTGGGTTAGACAATGAACCTGG 1378 48.11 56.29 61.39 25.57 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_53 CTGTGGGTTAGACAATGAACCTGGA 1379 49.51 57.52 62.52 27.17 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_53 CTGTGGGTTAGACAATGAACCTGGA 1379 49.51 57.52 62.52 27.17 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_54 TGTGGGTTAGACAATGAACCTGGAT 1380 46.90 55.19 60.38 26.35 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_54 TGTGGGTTAGACAATGAACCTGGAT 1380 46.90 55.19 60.38 26.35 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_55 TGGGTTAGACAATGAACCTGGATCG 1382 47.42 55.83 61.07 22.43 100.00% 0.00% - Reverse_N3_S1_1 TCTGTTACTTGGGCATAAAC 1412 43.12 49.82 53.57 23.17 100.00% 0.00% - 100.00% 0.00% Forward_N3_S1_55 TGGGTTAGACAATGAACCTGGATCG 1382 47.42 55.83 61.07 22.43 100.00% 0.00% - Reverse_N3_S1_2 TCTGTTACTTGGGCATAAACC 1411 46.24 52.45 55.85 28.40 100.00% 0.00% - 100.00% 0.00% Pairs: 113 Statistics Total candidates: 160 Pass degeneracy 160 (100.00 %) Pass GC Content 160 (100.00 %) Pass GC Clamp 160 (100.00 %) Pass Max Poly-X 160 (100.00 %) Pass self complementarity 160 (100.00 %) Pass self melting 160 (100.00 %) Failed self melting low 0 (0.00 %) Failed self melting high 0 (0.00 %) Pass targets 111 (69.38 %) Pass non targets 106 (66.25 %) Pass all tests 106 (66.25 %)