Primer Hunter
New Users:
Load your targets file and (optional) non targets file in fasta format in the corresponding boxes and click on the design primers button.
New Users:
Load your primers summary file in the primers file box and click on the design primers button.
Optionally, if you want to be notified as soon as the process is done, write at the end an email address where you want the results to be sent.
Read the
documentation
for further information.
Go to refilter primers
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Go back to design primers
Targets file
(Max 128M)
Sample
Non Targets file
(Max 128M)
Sample
Primers file
(Max 128M)
Sample
Min Primer Length
Max Primer Length
Min Product Length
Max Product Length
Forward Primer (5'-3')
Reverse Primer (5'-3')
Range of positions for forward primers
Begin:
End:
Range of positions for reverse primers
Begin:
End:
Targets Mask (3'-5')
Non Targets Mask (3'-5')
Degeneracy Mask (3'-5')
Number of Source Sequences
Minimum Targets Coverage (%)
Maximum Non Targets Coverage (%)
Max self score
Max end score
Min GC Content
Max GC Content
GC Clamp
Max Poly-X
Primer concentration (M)
Template Concentration (M)
Salt Concentration (M)
Salt Correction Method
Santalucia
Owczarzy
Targets Min Melting Temp (C)
Targets Max Melting Temp (C)
Non Targets Max Melting Temp (C)
Non Targets Delta Melting Temp (C)
Max Pair Tm Diff (C)
Primers Label
Full stats
E-mail address